Mapping genetic interactions in human cancer cells with RNAi and multiparametric phenotyping Christina Laufer, Bernd Fischer, Maximilian Billman, Wolfgang Huber, and Michael Boutros. Nature Methods (2013) 10(5):427-31 doi: 10.1038/nmeth.2436
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چکیده
5 Convert data from plate order to multi-dimensional SGI-arrary 11 5.1 Preliminaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5.2 Parse plate barcodes to annotate the plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5.3 Reorder data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
منابع مشابه
Mapping genetic interactions in human cancer cells with RNAi and multiparametric phenotyping Christina Laufer, Bernd Fischer, Maximilian Billmann, Wolfgang Huber, and Michael Boutros. Nature Methods (2013) 10(5):427-31 doi: 10.1038/nmeth.2436
5 Convert data from plate order to multi-dimensional SGI-arrary 11 5.1 Preliminaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5.2 Parse plate barcodes to annotate the plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5.3 Reorder data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....
متن کاملSource data and code for the paper A Map of Directional Genetic Interactions in a Metazoan Cell Bernd Fischer, Thomas Sandmann, Thomas Horn, Maximilian Billman, Varun Chaudhary, Wolfgang Huber, and Michael Boutros eLife, in press, http://www.elifesciences.org
6 Quality control 10 6.1 Quality control of features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 6.2 Quality control of dsRNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 6.3 Discussion of feature and target gene reagent quality control . . . . . . . . . . . . . . . . . . . . . . 13 6.4 Comparison to Rohn et al. . . ...
متن کاملExperimental data and documented source code for the paper A Map of Directional Genetic Interactions in a Metazoan Cell Bernd Fischer, Thomas Sandmann, Thomas Horn, Maximilian Billmann, Varun Chaudhary, Wolfgang Huber, and Michael Boutros eLife, in press, http://www.elifesciences.org
6 Quality control 10 6.1 Quality control of features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 6.2 Quality control of dsRNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 6.3 Discussion of feature and target gene reagent quality control . . . . . . . . . . . . . . . . . . . . . . 13 6.4 Comparison to Rohn et al. . . ...
متن کاملClustering phenotype populations by genome-wide RNAi and multiparametric imaging
Genetic screens for phenotypic similarity have made key contributions to associating genes with biological processes. With RNA interference (RNAi), highly parallel phenotyping of loss-of-function effects in cells has become feasible. One of the current challenges however is the computational categorization of visual phenotypes and the prediction of biological function and processes. In this stu...
متن کاملA map of directional genetic interactions in a metazoan cell
Gene-gene interactions shape complex phenotypes and modify the effects of mutations during development and disease. The effects of statistical gene-gene interactions on phenotypes have been used to assign genes to functional modules. However, directional, epistatic interactions, which reflect regulatory relationships between genes, have been challenging to map at large-scale. Here, we used comb...
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تاریخ انتشار 2013